| First Author | Liu SH | Year | 1995 |
| Journal | Dev Genet | Volume | 16 |
| Issue | 3 | Pages | 229-40 |
| PubMed ID | 7796532 | Mgi Jnum | J:25277 |
| Mgi Id | MGI:73002 | Doi | 10.1002/dvg.1020160303 |
| Citation | Liu SH, et al. (1995) Serum response element associated transcription factors in mouse embryos: serum response factor, YY1, and PEA3 factor. Dev Genet 16(3):229-40 |
| abstractText | Many mammalian transcription factors, including human and mouse serum response factors (SRFs), are post-translationally modified with O-linked N-acetylglucosamine monosaccharides on multiple serine and/or threonine residues. Nuclear extracts were prepared from 9.5 to 19 days postcoitum mouse embryos and subsequently were fractionated by wheat germ agglutinin (WGA)-agarose affinity chromatography. SRF binds WGA-agarose and apparently is O-glycosylated. On the other hand, the low molecular weight serum response element (SRE)-binding proteins, including the previously named band I and band II factors, did not bind WGA-agarose. Furthermore, we showed that the fastest migrating complex contains the Yin-Yang 1 (YY1) factor. YY1 binds to the c-fos SRE and skeletal alpha-actin muscle regulatory element (MRE), but not the cardiac alpha-actin MRE. Nuclear extracts from NIH/3T3 fibroblasts contain similar, if not identical, SRE-binding complexes. Besides these SRE-binding factors, mouse PEA3-binding factor, presumably an ETS domain-containing protein, was found to bind SRF protein. This physical interaction, between SRF and ETS domain proteins, was shown to involve the DNA-binding domain-containing region of SRF and not the carboxyl-terminal transactivation domain. |
