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Publication : Genomic analysis using a yeast artificial chromosome library with mouse DNA inserts.

First Author  Rossi JM Year  1992
Journal  Proc Natl Acad Sci U S A Volume  89
Issue  6 Pages  2456-60
PubMed ID  1347950 Mgi Jnum  J:67
Mgi Id  MGI:48607 Doi  10.1073/pnas.89.6.2456
Citation  Rossi JM, et al. (1992) Genomic analysis using a yeast artificial chromosome library with mouse DNA inserts. Proc Natl Acad Sci U S A 89(6):2456-60
abstractText  A yeast artificial chromosome library with mouse genomic DNA inserts has been constructed. The library encompasses a 2.5-fold coverage of the mouse genome, with an average insert size of 250 kilobases. The screening strategy uses the polymerase chain reaction on pooled DNAs prepared from individually stored clones. The usefulness of the library for chromosome walking was illustrated by constructing a 600-kilobase-long contig of DNA surrounding Hba-ps4, a DNA marker that is tightly linked to the fused (Fu) locus on chromosome 17.
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