| Primary Identifier | MGI:4836382 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence | Gene | Grhl3 |
| Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An 1808 bp cDNA fragment of the mouse Grhl2 gene was inserted such that the codon 12 of exon 2 of Grhl3 was fused to the corresponding codon of Grhl2. This insertion replaced part of exon 2 and all of intron 3 and exon 3. In addition a floxed neo cassette was inserted after the cDNA and then removed by Cre mediated recombination. RT-PCR analysis confirmed the expression of the fusion gene in homozygous and heterozygous mice and absence of expression of the endogenous gene in homozygous mice. |
