| Primary Identifier | MGI:5882209 | Allele Type | Targeted |
| Attribute String | Constitutively active | Gene | Xist |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A CAG promoter and a loxP site flanked puromycin resistance gene and ECFP reporter gene double cassette were inserted upstream of exon 1 replacing the native promoter. Subsequent cre-mediated recombination removed the selection/reporter cassette, bringing the gene under the constitutively active control of the CAG promoter. The expression from this allele is around tenfold higher than from the wild-type allele. The half-life of the transcripts is the same as wild-type. |
