| Primary Identifier | MGI:6191780 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Ngb |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 87146546 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 87148064. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was then created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. |
