| Primary Identifier | MGI:5904492 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Mtfmt |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | KOMP-CSD |
| molecularNote | The L1L2_gt2 cassette was inserted at position 65347199 of Chromosome 9 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 65347986. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). In vivo, a single T to C mutation at position 4 of the central spacer region in the distal (3') loxP site was found, which completely inhibited the recombination reaction in two conditional mouse models. High levels of cre expression in vitro could result in recombination. |
