Primary Identifier | MGI:7515019 | Allele Type | Targeted |
Attribute String | Modified isoform(s), Reporter | Gene | Foxp3 |
Transmission | Germline | Strain of Origin | (C57BL/6 x 129S/SvEv)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A targeting vector was constructed such that a Foxp3 cDNA lacking exons 2 and 7 was inserted at the translation initiation ATG codon of Foxp3 and the TGA stop of Foxp3 was replaced by a 2A peptide-GFP cassette followed by a double polyadenylation signal (pA) and an FRT-flanked neomycin cassette. The neomycin cassette was removed via flp-mediated recombination. |