| First Author | Liu CH | Year | 2004 |
| Journal | Reprod Domest Anim | Volume | 39 |
| Issue | 1 | Pages | 19-24 |
| PubMed ID | 15129916 | Mgi Jnum | J:91328 |
| Mgi Id | MGI:3046488 | Doi | 10.1046/j.1439-0531.2003.00470.x |
| Citation | Liu CH, et al. (2004) Stored of Hsp72/Hsp73 in germinal vesicle-stage mouse oocytes. Reprod Domest Anim 39(1):19-24 |
| abstractText | Heat-shock proteins (hsps) Hsp72 and Hsp73 are the stored maternal proteins found in mouse oocytes. Both hsps appear in mouse oocytes at germinal vesicle (GV) and metaphase II (M-II)-stages as previously demonstrated by immunoblotting analysis. In this report, we further determined the presences of Hsp72/Hsp73 proteins in mouse embryos at stages of 2-pronucleus, arrested 1-cell, 2-cell, arrested 2-cell, 4-cell, arrested 4-cell, 8-cell to morula and blastocyst. Except for the blastocyst stage, the Hsp72/Hsp73 proteins were detectable in most embryo stages. The concentration of Hsp72/Hsp73 in GV-stage oocytes was higher than that in M-II-stage oocytes, and in any stages of embryos before implantation. A dramatical increase in Hsp72/Hsp73 expression was found at the 2-cell stage. Together with these findings, we speculated that hsps accumulated or stored earlier in the GV-stage mouse oocytes to protect the oocytes against environmental influences acting on ovary, and hsps may be required for zygotic gene activation and provided a protective effect against apoptosis. |
