| First Author | Jin M | Year | 2002 |
| Journal | Genomics | Volume | 79 |
| Issue | 5 | Pages | 686-92 |
| PubMed ID | 11991718 | Mgi Jnum | J:76483 |
| Mgi Id | MGI:2179573 | Doi | 10.1006/geno.2002.6764 |
| Citation | Jin M, et al. (2002) A novel, brain-specific mouse drebrin: cDNA cloning, chromosomal mapping, genomic structure, expression, and functional characterization. Genomics 79(5):686-92 |
| abstractText | Drebrin A, a major neuronal actin-binding protein, regulates the dendritic spine shapes of neurons. Here, we have cloned and characterized a novel mouse cDNA clone encoding a truncated form of drebrin A, named s-drebrin A. Analysis of the genomic organization of the mouse drebrin gene (Dbn1), which mapped to the central portion of chromosome 13, revealed that isoforms including s-drebrin A are generated by alternative splicing from a single drebrin gene. The s-drebrin A mRNA was expressed in the brain, but not in non-neuronal tissues. The s-drebrin A expression was barely detected in the embryonic brain, but was upregulated during postnatal development of the brain. Overexpression of GFP-tagged s-drebrin A in fibroblasts showed it to be associated with actin filaments and with changes in actin cytoskeleton organization. These findings suggest that s-drebrin A has a role in spine morphogenesis, possibly by competing the actin-binding activity with drebrin A. (c)2002 Elsevier Science (USA). |
