| First Author | Wang ZG | Year | 2010 |
| Journal | Biochem J | Volume | 428 |
| Issue | 3 | Pages | 347-54 |
| PubMed ID | 20415667 | Mgi Jnum | J:163041 |
| Mgi Id | MGI:4820931 | Doi | 10.1042/BJ20100379 |
| Citation | Wang ZG, et al. (2010) Characterization of novel VEGF (vascular endothelial growth factor)-C splicing isoforms from mouse. Biochem J 428(3):347-54 |
| abstractText | VEGF (vascular endothelial growth factor)-C is a major growth factor implicated in various physiological processes, such as angiogenesis and lymphangiogenesis. In the present paper, we report the identification of three short VEGF-C splicing isoforms (VEGF-C62, VEGF-C129 and VEGF-C184) from immortalized mouse kidney PTECs (proximal tubular epithelial cells). Semi-quantitative RT (reverse transcription)-PCR analysis showed these isoforms were universally expressed to varying degrees in different tissues with high expression levels in the kidney. In immortalized PTECs and podocytes, VEGF-C62 can activate phosphorylation of FAK (focal adhesion kinase) and promote cell adhesion to substratum. Cell survival was also increased by VEGF-C62 treatment in the absence of serum. VEGF-C62 can also reduce cell proliferation in PTECs and podocytes. Nucleolin was one of the proteins that associated with VEGF-C62 in pull-down assays using GST (glutathione transferase) fusion proteins as bait, indicating different protein binding requirements for VEGF-C62 compared with VEGF-C. In conclusion, these newly identified VEGF-C isoforms represent a new class of proteins, which are potentially involved in epithelial cell adhesion and proliferation through novel receptor pathways. |
