Primary Identifier | MGI:5897983 | Allele Type | Targeted |
Attribute String | Not Specified | Gene | Cdh23 |
Inheritance Mode | Dominant | Transmission | Germline |
Strain of Origin | C57BL/6N-A<tm1Brd> | Is Recombinase | false |
Is Wild Type | false |
molecularNote | Chr10: 60,527,162 bp - 60,536,889 bp (GRCm38), which contains the functional c.753G variant, was taken from a 129S1-derived BAC, a loxP-flanked PGK-Neo cassette was inserted 178 bp downstream of the c.753 nucleotide, homology arms were added to correctly target this sequence to replace the endogenous coding sequence via homologous recombination in C57BL/6N-derived ES cells, resulting in a A to G point mutation, which effectively reverts the ahl allele to wild-type, with a downstream loxP-flanked PGK-Neo cassette. |