| First Author | Morii E | Year | 2001 |
| Journal | Blood | Volume | 98 |
| Issue | 8 | Pages | 2577-9 |
| PubMed ID | 11588059 | Mgi Jnum | J:131110 |
| Mgi Id | MGI:3772977 | Doi | 10.1182/blood.v98.8.2577 |
| Citation | Morii E, et al. (2001) Effect of a large deletion of the basic domain of mi transcription factor on differentiation of mast cells. Blood 98(8):2577-9 |
| abstractText | The mi transcription factor (MITF) is a basic-helix-loop-helix-leucine zipper transcription factor that is important for the development of mast cells. Cultured mast cells (CMCs) of mi/mi genotype express abnormal MITF (mi-MITF), but CMCs of tg/tg genotype do not express any MITFs. It was previously reported that mi/mi CMCs showed more severe abnormalities than tg/tg CMCs, indicating that mi-MITF had inhibitory function. Whereas mi-MITF contains a single amino acid deletion in the basic domain, MITF encoded by mi(ew) allele (ew-MITF) deletes 16 of 21 amino acids of the basic domain. Here the effect of a large deletion of the basic domain was examined. In mi(ew)/mi(ew) CMCs, the expression pattern of genes whose transcription was affected by MITF was comparable to that of tg/tg CMCs rather than to that of mi/mi CMCs. This suggested that ew-MITF lacked any functions. The part of the basic domain deleted in ew-MITF appeared necessary for either transactivation or inhibition of transactivation. |
