| First Author | Marchetto GS | Year | 1995 |
| Journal | Gene | Volume | 158 |
| Issue | 2 | Pages | 303-4 |
| PubMed ID | 7607559 | Mgi Jnum | J:26497 |
| Mgi Id | MGI:73943 | Doi | 10.1016/0378-1119(95)00118-p |
| Citation | Marchetto GS, et al. (1995) Cloning and sequencing of the cDNA encoding the avian kidney cAMP-dependent protein kinase inhibitor protein. Gene 158(2):303-4 |
| abstractText | The endogenous inhibitor of cAMP-dependent protein kinase (PKA) is down-regulated in the kidneys from vitamin-D-replete chicks as compared to vitamin-D-deficient chicks. Screening of a vitamin-D-deficient chick kidney library resulted in the isolation of a 450-bp cDNA clone encoding the 76-amino acid (aa) protein kinase inhibitor (PKI). The deduced aa sequence of avian PKI shares 80 and 41% identity with the mammalian PKI alpha and PKI beta 1 isoforms, respectively. The chick and mammalian PKI contain conserved N-terminal sequences, including the pseudo-substrate site (18GRRNA22), which are required for potent inhibition of the catalytic subunit of PKA. Chick kidney PKI contains ten unique aa in the C-terminal portion of the protein that are not shared with the mammalian PKI alpha or beta isoforms. |
