| First Author | Wang Y | Year | 2003 |
| Journal | Genomics | Volume | 81 |
| Issue | 2 | Pages | 126-37 |
| PubMed ID | 12620390 | Mgi Jnum | J:82469 |
| Mgi Id | MGI:2653377 | Doi | 10.1016/s0888-7543(02)00024-1 |
| Citation | Wang Y, et al. (2003) Genomic definition of RIM proteins: evolutionary amplification of a family of synaptic regulatory proteins( small star, filled ). Genomics 81(2):126-37 |
| abstractText | RIMs are synaptic proteins that are essential for normal neurotransmitter release. We now show that while invertebrates contain only a single RIM gene, vertebrates contain four: two large genes encoding RIM1alpha (0.50 Mb) or RIM2alpha, 2beta, and 2gamma (0.50-0.75 Mb) and two smaller genes encoding RIM3gamma (14 kb) or RIM4gamma (55 kb). RIM1alpha and RIM2alpha consist of an N-terminal Zn(2+)-finger domain, central PDZ and C(2)A domains, and a C-terminal C(2)B domain; RIM2beta consists of a short beta-specific sequence followed by central PDZ and C(2)A domains and a C-terminal C(2)B domain; and RIM2gamma, 3gamma, and 4gamma consist of only a C(2)B domain. In the RIM2 gene, RIM2beta and 2gamma are transcribed from internal promoters. alpha- and beta-RIMs are extensively alternatively spliced at three canonical positions, resulting in >200 variants that differ by up to 400 residues. Thus gene duplication, alternative splicing, and multiple promoters diversify a single invertebrate RIM into a large vertebrate protein family. The multiplicity of vertebrate RIMs may serve to fine-tune neurotransmitter release beyond a fundamental, evolutionarily conserved, and common function for RIMs. |
