| First Author | Mariño G | Year | 2007 |
| Journal | J Biol Chem | Volume | 282 |
| Issue | 25 | Pages | 18573-83 |
| PubMed ID | 17442669 | Mgi Jnum | J:123386 |
| Mgi Id | MGI:3718179 | Doi | 10.1074/jbc.M701194200 |
| Citation | Marino G, et al. (2007) Tissue-specific autophagy alterations and increased tumorigenesis in mice deficient in Atg4C/autophagin-3. J Biol Chem 282(25):18573-83 |
| abstractText | Atg4C/autophagin-3 is a member of a family of cysteine proteinases proposed to be involved in the processing and delipidation of the mammalian orthologues of yeast Atg8, an essential component of an ubiquitin-like modification system required for execution of autophagy. To date, the in vivo role of the different members of this family of proteinases remains unclear. To gain further insights into the functional relevance of Atg4 orthologues, we have generated mutant mice deficient in Atg4C/autophagin-3. These mice are viable and fertile and do not display any obvious abnormalities, indicating that they are able to develop the autophagic response required during the early neonatal period. However, Atg4C-/--starved mice show a decreased autophagic activity in the diaphragm as assessed by immunoblotting studies and by fluorescence microscopic analysis of samples from Atg4C-/- GFP-LC3 transgenic mice. In addition, animals deficient in Atg4C show an increased susceptibility to develop fibrosarcomas induced by chemical carcinogens. Based on these results, we propose that Atg4C is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation. We also propose that this enzyme could play an in vivo role in events associated with tumor progression. <br>This article has been withdrawn by the authors upon request from the Journal. The Journal raised questions regarding Fig.1C. In Fig. 1C, actin lanes appeared to be duplicated, and the original data for this panel were not available. The authors state that a new experiment was performed using RNA from mouse tissues in WT and Atg4C-deficient animals. The authors assert that all of the results reported in this article are valid, some of which are supported by a subsequent report by another group (Li et al. (2011) J. Biol. Chem. 286, 7327). |
