Primary Identifier | MGI:6877060 | Allele Type | Endonuclease-mediated |
Attribute String | Epitope tag, Humanized sequence, Inserted expressed sequence | Gene | Gt(ROSA)26Sor |
Strain of Origin | C57BL/6J | Is Recombinase | false |
Is Wild Type | false |
molecularNote | CRISPR/cas9 genome editing and RMCE (recombinase-mediated cassette exchange) are utilized to insert a human keratin 18 promoter directing expression of human ACE2 followed a C-terminal FLAG epitope tag into a modified Gt(ROSA)26Sor (B6.RosaBxb-GT/GA) locus. The C57BL/6J-derived zygotes carrying the Gt(ROSA)26Sor |