| First Author | Gribaudo G | Year | 1997 |
| Journal | Eur J Biochem | Volume | 249 |
| Issue | 1 | Pages | 258-64 |
| PubMed ID | 9363777 | Mgi Jnum | J:43724 |
| Mgi Id | MGI:1098385 | Doi | 10.1111/j.1432-1033.1997.t01-1-00258.x |
| Citation | Gribaudo G, et al. (1997) Molecular cloning and expression of an interferon-inducible protein encoded by gene 203 from the gene 200 cluster. Eur J Biochem 249(1):258-64 |
| abstractText | We report here the complete coding sequence of a 203 cDNA, a member of the interferon-inducible Ifi 200 gene family. By combining reverse-transcriptase PCR and rapid amplification of cDNA ends (RACE) techniques we have obtained a 3.8-kb cDNA corresponding to a 203 mRNA. When used as a probe in northern analysis, its 3' segment hybridized to a 3.8-kb interferon-inducible mRNA, whereas the 5'-end additionally hybridized to a less abundant interferon-inducible 1.8-kb mRNA. Nucleotide sequence analysis revealed that the two mRNAs share the 5'-untranslated region and the same open reading frame, which encodes a hydrophilic protein composed of 408 amino acids. The difference between them is due to a 3'-untranslated region extended by alternative polyadenylation site selection. Furthermore, 203 mRNA was found to be inducible by interferon-alpha in various murine cell lines. Using polyclonal antibodies raised against a segment specific for the 203 protein, we established that p203 protein levels increase on treatment with interferon-alpha in murine fibroblasts and that p203 is located in the nucleus. |
