| First Author | Yuba-Kubo A | Year | 2005 |
| Journal | Dev Biol | Volume | 282 |
| Issue | 2 | Pages | 361-73 |
| PubMed ID | 15893303 | Mgi Jnum | J:99398 |
| Mgi Id | MGI:3582102 | Doi | 10.1016/j.ydbio.2005.03.031 |
| Citation | Yuba-Kubo A, et al. (2005) Gene knockout analysis of two gamma-tubulin isoforms in mice. Dev Biol 282(2):361-73 |
| abstractText | gamma-Tubulin regulates the nucleation of microtubules, but knowledge of its functions in vivo is still fragmentary. Here, we report the identification of two closely related gamma-tubulin isoforms, TUBG1 and TUBG2, in mice, and the generation of TUBG1- and TUBG2-deficient mice. TUBG1 was expressed ubiquitously, whereas TUBG2 was primarily detected in the brain. The development of TUBG1-deficient (Tubg1(-/-)) embryos stopped at the morula/blastocyst stages due to a characteristic mitotic arrest: the mitotic spindle was highly disorganized, and disorganized spindles showed one or two pole-like foci of bundled MTs that were surrounded by condensed chromosomes. TUBG2 was expressed in blastocysts, but could not rescue the TUBG1 deficiency. By contrast, TUBG2-deficient (Tubg2(-/-)) mice were born, grew, and intercrossed normally. In the brain of wild-type mice, TUBG2 was expressed in approximately the same amount as TUBG1, but no histological abnormalities were found in the Tubg2(-/-) brain. These findings indicated that TUBG1 and TUBG2 are not functionally equivalent in vivo, that TUBG1 corresponds to conventional gamma-tubulin, and that TUBG2 may have some unidentified function in the brain. |
