| First Author | Seiriki K | Year | 2017 |
| Journal | Neuron | Volume | 94 |
| Issue | 6 | Pages | 1085-1100.e6 |
| PubMed ID | 28641108 | Mgi Jnum | J:256136 |
| Mgi Id | MGI:6114562 | Doi | 10.1016/j.neuron.2017.05.017 |
| Citation | Seiriki K, et al. (2017) High-Speed and Scalable Whole-Brain Imaging in Rodents and Primates. Neuron 94(6):1085-1100.e6 |
| abstractText | Subcellular resolution imaging of the whole brain and subsequent image analysis are prerequisites for understanding anatomical and functional brain networks. Here, we have developed a very high-speed serial-sectioning imaging system named FAST (block-face serial microscopy tomography), which acquires high-resolution images of a whole mouse brain in a speed range comparable to that of light-sheet fluorescence microscopy. FAST enables complete visualization of the brain at a resolution sufficient to resolve all cells and their subcellular structures. FAST renders unbiased quantitative group comparisons of normal and disease model brain cells for the whole brain at a high spatial resolution. Furthermore, FAST is highly scalable to non-human primate brains and human postmortem brain tissues, and can visualize neuronal projections in a whole adult marmoset brain. Thus, FAST provides new opportunities for global approaches that will allow for a better understanding of brain systems in multiple animal models and in human diseases. |
