| First Author | Hermann M | Year | 2014 |
| Journal | Nucleic Acids Res | Volume | 42 |
| Issue | 6 | Pages | 3894-907 |
| PubMed ID | 24413561 | Mgi Jnum | J:231986 |
| Mgi Id | MGI:5775694 | Doi | 10.1093/nar/gkt1361 |
| Citation | Hermann M, et al. (2014) Binary recombinase systems for high-resolution conditional mutagenesis. Nucleic Acids Res 42(6):3894-907 |
| abstractText | Conditional mutagenesis using Cre recombinase expressed from tissue specific promoters facilitates analyses of gene function and cell lineage tracing. Here, we describe two novel dual-promoter-driven conditional mutagenesis systems designed for greater accuracy and optimal efficiency of recombination. Co-Driver employs a recombinase cascade of Dre and Dre-respondent Cre, which processes loxP-flanked alleles only when both recombinases are expressed in a predetermined temporal sequence. This unique property makes Co-Driver ideal for sequential lineage tracing studies aimed at unraveling the relationships between cellular precursors and mature cell types. Co-InCre was designed for highly efficient intersectional conditional transgenesis. It relies on highly active trans-splicing inteins and promoters with simultaneous transcriptional activity to reconstitute Cre recombinase from two inactive precursor fragments. By generating native Cre, Co-InCre attains recombination rates that exceed all other binary SSR systems evaluated in this study. Both Co-Driver and Co-InCre significantly extend the utility of existing Cre-responsive alleles. |
