| First Author | Morton G | Year | 2018 |
| Journal | J Neurosci | PubMed ID | 29954848 |
| Mgi Jnum | J:264332 | Mgi Id | MGI:6194823 |
| Doi | 10.1523/JNEUROSCI.0023-18.2018 | Citation | Morton G, et al. (2018) Chrna5-expressing neurons in the interpeduncular nucleus mediate aversion primed by prior stimulation or nicotine exposure. J Neurosci |
| abstractText | Genetic studies have shown an association between smoking and variation at the CHRNA5/A3/B4 gene locus, encoding the alpha5, alpha3 and beta4 nicotinic receptor subunits. The alpha5 receptor has been specifically implicated because smoking-associated haplotypes contain a coding variant in the CHRNA5 gene. The Chrna5/a3/b4 locus is conserved in rodents, and the restricted expression of these subunits suggests neural pathways through which the reinforcing and aversive properties of nicotine may be mediated. Here we show that in the interpeduncular nucleus (IP), the site of the highest Chrna5 mRNA expression in rodents, electrophysiological responses to nicotinic acetylcholine receptor stimulation are markedly reduced in alpha5 null mice. In this regard, we find IP neurons differ markedly from their upstream ventral medial habenula cholinergic partners, which appear unaltered by loss of alpha5. To probe the functional role of alpha5-containing IP neurons, we used BAC recombineering to generate transgenic mice expressing Cre-recombinase from the Chrna5 locus. Reporter expression driven by Chrna5(Cre) demonstrates that transcription of Chrna5 is regulated independently from the Chrna3/b4 genes, transcribed on the opposite strand. Chrna5-expressing IP neurons are GABAergic and project to distant targets in the mesopontine raphe and tegmentum, rather than forming local circuits. Optogenetic stimulation of Chrna5-expressing IP neurons failed to elicit physical manifestations of withdrawal. However, following recent prior stimulation or exposure to nicotine, IP stimulation becomes aversive. These results using mice of both sexes support the idea that the risk allele of CHRNA5 may increase the drive to smoke via loss of IP-mediated nicotine aversion.SIGNIFICANCE STATEMENTUnderstanding the receptors and neural pathways underlying the reinforcing and aversive effects of nicotine may suggest new treatments for tobacco addiction. Part of the individual variability in smoking is associated with specific forms of the alpha5 nicotinic receptor subunit gene. Here we show that deletion of the alpha5 subunit in mice markedly reduces the cellular response to nicotine and acetylcholine in the interpeduncular nucleus (IP). Stimulation of alpha5-expressing IP neurons using optogenetics is aversive, but this effect requires priming by recent prior stimulation or exposure to nicotine.. These results support the idea that the smoking-associated variant of the alpha5 gene may increase the drive to smoke via loss of IP-mediated nicotine aversion. |
