| First Author | Sandrin MS | Year | 1992 |
| Journal | J Immunol | Volume | 149 |
| Issue | 5 | Pages | 1636-41 |
| PubMed ID | 1506686 | Mgi Jnum | J:2074 |
| Mgi Id | MGI:50598 | Doi | 10.4049/jimmunol.149.5.1636 |
| Citation | Sandrin MS, et al. (1992) Isolation and characterization of cDNA clones for mouse Ly-9. J Immunol 149(5):1636-41 |
| abstractText | We describe the production and characterization of a mouse mAb, S-450-33.2, recognizing the Ly-9.2 specificity. This mAb was used to purify Ly-9 molecules from lymphoid cell lines, and the amino-terminal amino acids were determined. The mAb was also used in a eukaryotic expression system, to isolate cDNA clones encoding Ly-9. Analysis of RNA showed that Ly-9 expression is lymphocyte specific, as determined by the presence of a single hybridizing 2.4-kb species found only in lymphoid cells. Genomic DNA analysis indicated that Ly-9 is encoded by a single-copy gene of 10 to 15 kb. The predicted polypeptide belongs to the Ig superfamily of cell surface molecules with four extracellular Ig-like domains, i.e., a non-disulfide-bonded V domain, a truncated C2 domain with two disulfide bonds, a second non-disulfide-bonded V domain, and a truncated C2 domain with two disulfide bonds (V-C2-V-C2). The sequence data also support the view that Ly-9 belongs to the subgroup of the Ig superfamily that includes Bcm-1, CD2, and LFA-3. |
