| First Author | Fisher KJ | Year | 1992 |
| Journal | Mol Cell Biol | Volume | 12 |
| Issue | 4 | Pages | 1585-91 |
| PubMed ID | 1549114 | Mgi Jnum | J:14618 |
| Mgi Id | MGI:62782 | Doi | 10.1128/mcb.12.4.1585 |
| Citation | Fisher KJ, et al. (1992) Characterization of the cDNA and genomic sequence of a G protein gamma subunit (gamma 5). Mol Cell Biol 12(4):1585-91 |
| abstractText | A cDNA from human placenta and liver tissues that contained both sequence for the lysosomal glycosidase di-N-acetylchitobiase and sequence homologous to the gamma subunit of GTP-binding proteins was previously isolated. Here we have shown that the gamma-subunit-homologous portion of this unusual cDNA is derived from a member of the gamma-subunit multigene family. The partial human gamma-subunit sequence was used to isolate the corresponding full-length cDNA clones from bovine and rat livers. The two cDNAs encode identical 68-amino-acid proteins (7.3 kDa) homologous to previously cloned G protein gamma subunits. The bovine gene sequence encoding this new gamma-subunit isoform (gamma 5) was determined and found to have an intron-exon structure consistent with the original human chitobiase-gamma 5-subunit hybrid mRNA being a product of alternative splicing. Genomic cloning also resulted in the isolation of a human gamma 5 pseudogene. |
