| First Author | Silveira MA | Year | 2017 |
| Journal | Mol Cell Endocrinol | Volume | 448 |
| Pages | 55-65 | PubMed ID | 28344041 |
| Mgi Jnum | J:251145 | Mgi Id | MGI:6104111 |
| Doi | 10.1016/j.mce.2017.03.024 | Citation | Silveira MA, et al. (2017) STAT5 signaling in kisspeptin cells regulates the timing of puberty. Mol Cell Endocrinol 448:55-65 |
| abstractText | Previous studies have shown that kisspeptin neurons are important mediators of prolactin's effects on reproduction. However, the cellular mechanisms recruited by prolactin to affect kisspeptin neurons remain unknown. Using whole-cell patch-clamp recordings of brain slices from kisspeptin reporter mice, we observed that 20% of kisspeptin neurons in the anteroventral periventricular nucleus was indirectly depolarized by prolactin via an unknown population of prolactin responsive neurons. This effect required the phosphatidylinositol 3-kinase signaling pathway. No effects on the activity of arcuate kisspeptin neurons were observed, despite a high percentage (70%) of arcuate neurons expressing prolactin-induced STAT5 phosphorylation. To determine whether STAT5 expression in kisspeptin cells regulates reproduction, mice carrying Stat5a/b inactivation specifically in kisspeptin cells were generated. These mutants exhibited an early onset of estrous cyclicity, indicating that STAT5 transcription factors exert an inhibitory effect on the timing of puberty. |
