Primary Identifier | MGI:6280488 | Allele Type | Targeted |
Attribute String | Conditional ready, Humanized sequence, Inserted expressed sequence, Null/knockout, Reporter | Gene | Hip1 |
Transmission | Germline | Strain of Origin | C57BL/6 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The targeting vector contains a floxed STOP cassette (LSL) inserted into intron 1, followed by a partial exon 2 fused to a human HIP1 (huntingtin interacting protein 1) cDNA, IRES-EGFP, and a polyadenylation signal sequence. An FRT-flanked neomycin cassette inserted after the poly(A) was removed by a Flp-expressing plasmid. Western blot analysis on lung tissue confirmed the absence of mouse Hip1 protein. |