| Primary Identifier | MGI:5303433 | Allele Type | Targeted |
| Gene | Hprt1 | Transmission | Cell Line |
| Strain of Origin | (B6.129P2-Hprt1<b-m3>/J x 129S-Gt(ROSA)26Sor<tm1Sor>/J)F1 | Is Recombinase | false |
| Is Wild Type | false | Project Collection | Pleiades Promoter Project |
| molecularNote | The Ple139-lacZ plasmid (pEMS1611) was created by inserting the Ple139 MiniPromoter (derived from specific promoter/enhancer/regulatory regions of the human nuclear receptor subfamily 2, group E, member 1 (NR2E1) gene) into the multiple cloning site of the pEMS1313 plasmid vector backbone. This placed the Ple139 MiniPromoter upstream of an intron, a full F5 mutant-frt site, a Kozak consensus sequence, a beta-galactosidase (lacZ) gene, a full wildtype frt site, an SV40 early polyA signal, a human HPRT complementary sequence (containing exon 1, intron 1, exon 2, and part of intron 2), a mouse 3' Hprt homology arm, an I-Sce linearization cut site, and a mouse 5' Hprt homology arm. This Ple139-lacZ construct was targeted as a single copy knock-in to the Hprtb-m3 mutant locus on the X Chromosome via electroporation into mEMS1202 embryonic stem cells. |
