| First Author | Lu KP | Year | 2003 |
| Journal | J Biol Chem | Volume | 278 |
| Issue | 30 | Pages | 28201-9 |
| PubMed ID | 12714586 | Mgi Jnum | J:84913 |
| Mgi Id | MGI:2670801 | Doi | 10.1074/jbc.M303888200 |
| Citation | Lu KP, et al. (2003) Redox regulation of a novel L1Md-A2 retrotransposon in vascular smooth muscle cells. J Biol Chem 278(30):28201-9 |
| abstractText | Activation and reintegration of retrotransposons into the genome is linked to several diseases in human and rodents, but mechanisms of gene activation remain largely unknown. Here we identify a novel gene of L1Md-A2 lineage in vascular smooth muscle cells and show that environmental hydrocarbons enhance gene expression and activate monomer-driven transcription via a redox-sensitive mechanism. Site-directed mutagenesis and progressive deletion analyses identified two antioxidant/electrophile response-like elements (5'-GTGACTCGAGC-3') within the A2/3 and A3 region. These elements mediated activation, with the A3 monomer playing an essential role in transactivation. This signaling pathway may contribute to gene instability during the course of atherogenesis. |
