Primary Identifier | MGI:5635821 | Allele Type | Targeted |
Attribute String | Inducible, Null/knockout, Recombinase, Reporter | Gene | Lgr5 |
Transmission | Germline | Strain of Origin | C57BL/6 |
Induced With | tamoxifen | Is Recombinase | true |
Is Wild Type | false |
molecularNote | The targeting construct replaced the first 22 amino acids with an EGFP reporter gene, IRES, inducible cre recombinase fusion (cre/ERT2), polyadenylation sequence and FRT-flanked neomycin resistance cassette. Flp-mediated recombination removed the selection cassette. In situ hybridization confirmed the absence of transcript expression in the intestin, skin and kidney. |