| First Author | Tartaglia LA | Year | 1995 |
| Journal | Cell | Volume | 83 |
| Issue | 7 | Pages | 1263-71 |
| PubMed ID | 8548812 | Mgi Jnum | J:30407 |
| Mgi Id | MGI:77918 | Doi | 10.1016/0092-8674(95)90151-5 |
| Citation | Tartaglia LA, et al. (1995) Identification and expression cloning of a leptin receptor, OB-R. Cell 83(7):1263-71 |
| abstractText | The ob gene product, leptin, is an important circulating signal for the regulation of body weight. To identify high affinity leptin-binding sites, we generated a series of leptin-alkaline phosphatase (AP) fusion proteins as well as [125I]leptin. After a binding survey of cell lines and tissues, we identified leptin-binding sites in the mouse choroid plexus. A cDNA expression library was prepared from mouse choroid plexus and screened with a leptin-AP fusion protein to identify a leptin receptor (OB-R). OB-R is a single membrane-spanning receptor most related to the gp130 signal-transducing component of the IL-6 receptor, the G-CSF receptor, and the LIF receptor. OB-R mRNA is expressed not only in choroid plexus, but also in several other tissues, including hypothalamus. Genetic mapping of the gene encoding OB-R shows that it is within the 5.1 cM interval of mouse chromosome 4 that contains the db locus. |
