| First Author | Lillien L | Year | 2014 |
| Journal | Dev Neurobiol | Volume | 74 |
| Issue | 11 | Pages | 1096-109 |
| PubMed ID | 24771701 | Mgi Jnum | J:242054 |
| Mgi Id | MGI:5904244 | Doi | 10.1002/dneu.22186 |
| Citation | Lillien L (2014) Rostral-caudal distribution of Emx1-lineage stem/transit amplifying cells and lineage progression in embryonic cortex depend on Hedgehog signaling. Dev Neurobiol 74(11):1096-109 |
| abstractText | Lineage progression of neural precursors to an EGF-responsive state can be promoted by several extrinsic signals, including fibroblast growth factor 2 (FGF2) and Hedgehog (Hh). It has been suggested that EGF-responsive precursors in the embryonic cerebral cortex originate in the ventral telencephalon in an FGF-dependent manner and migrate dorsally. To determine whether cortical EGF-responsive cells originate locally from dorsal precursors, we marked these precursors using Emx1-cre and the cre reporter Z/EG and observed a local origin for EGF-responsive cells. We also found a rostral-caudal difference in the abundance of self-renewing, neurogenic Emx1-lineage precursors, with more present rostrally. Deleting the Hh receptor smoothened in Emx-1 lineage cells impaired their progression to an EGF-responsive state. Moreover, loss of smoothened increased the proportion of neurogenic, self-renewing Emx1-lineage cells in caudal regions of cortex, eliminating their asymmetric distribution. Our results support the idea that Hh signaling promotes lineage progression of stem/transit amplifying cells, particularly in caudal regions of the embryonic cortex, leading to rostral-caudal differences in the abundance of neurogenic, self-renewing precursors. |
