| First Author | Sobajima T | Year | 2018 |
| Journal | J Cell Biol | Volume | 217 |
| Issue | 5 | Pages | 1777-1796 |
| PubMed ID | 29514919 | Mgi Jnum | J:261440 |
| Mgi Id | MGI:6155399 | Doi | 10.1083/jcb.201709123 |
| Citation | Sobajima T, et al. (2018) The Rab11-binding protein RELCH/KIAA1468 controls intracellular cholesterol distribution. J Cell Biol 217(5):1777-1796 |
| abstractText | Cholesterol, which is endocytosed to the late endosome (LE)/lysosome, is delivered to other organelles through vesicular and nonvesicular transport mechanisms. In this study, we discuss a novel mechanism of cholesterol transport from recycling endosomes (REs) to the trans-Golgi network (TGN) through RELCH/KIAA1468, which is newly identified in this study as a Rab11-GTP- and OSBP-binding protein. After treating cells with 25-hydroxycholesterol to induce OSBP relocation from the cytoplasm to the TGN, REs accumulated around the TGN area, but this accumulation was diminished in RELCH- or OSBP-depleted cells. Cholesterol content in the TGN was decreased in Rab11-, RELCH-, and OSBP-depleted cells and increased in the LE/lysosome. According to in vitro reconstitution experiments, RELCH tethers Rab11-bound RE-like and OSBP-bound TGN-like liposomes and promotes OSBP-dependent cholesterol transfer from RE-like to TGN-like liposomes. These data suggest that RELCH promotes nonvesicular cholesterol transport from REs to the TGN through membrane tethering. |
