Primary Identifier | MGI:2182943 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Efna2 |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A neomycin selection cassette, containing stop codons in all three reading frames, was inserted after the sequence encoding amino acid 66. The insertion was upstream of a conserved cysteine residue motif. RT-PCR analysis of midbrain RNA showed an absence of transcript in homozygous mutant embryos. |