| First Author | Liu G | Year | 2020 |
| Journal | Cell Rep | Volume | 33 |
| Issue | 12 | Pages | 108524 |
| PubMed ID | 33357428 | Mgi Jnum | J:300918 |
| Mgi Id | MGI:6489108 | Doi | 10.1016/j.celrep.2020.108524 |
| Citation | Liu G, et al. (2020) Direct Measurement of Cerebrospinal Fluid Production in Mice. Cell Rep 33(12):108524 |
| abstractText | The emerging interest in brain fluid transport has prompted a need for techniques that provide an understanding of what factors regulate cerebrospinal fluid (CSF) production. Here, we describe a methodology for direct quantification of CSF production in awake mice. We measure CSF production by placing a catheter in a lateral ventricle, while physically blocking outflow from the 4(th) ventricle. Using this methodology, we show that CSF production increases during isoflurane anesthesia, and to a lesser extent with ketamine/xylazine anesthesia, relative to the awake state. Aged mice have reduced CSF production, which is even lower in aged mice overexpressing amyloid-beta. Unexpectedly, CSF production in young female mice is 30% higher than in age-matched males. Altogether, the present observations imply that a reduction in CSF production might contribute to the age-related risk of proteinopathies but that the rate of CSF production and glymphatic fluid transport are not directly linked. |
