| First Author | Curtis PJ | Year | 1983 |
| Journal | J Biol Chem | Volume | 258 |
| Issue | 7 | Pages | 4459-63 |
| PubMed ID | 6187736 | Mgi Jnum | J:7016 |
| Mgi Id | MGI:55487 | Doi | 10.1016/s0021-9258(18)32645-0 |
| Citation | Curtis PJ (1983) Cloning of mouse carbonic anhydrase mRNA and its induction in mouse erythroleukemic cells. J Biol Chem 258(7):4459-63 |
| abstractText | Mouse carbonic anhydrase mRNA was detected in poly(A+) RNA of anemic spleens sedimenting as a RNA species at 14 S. Subsequently, poly(A+) RNA (12-16 S) was used as a template for the synthesis of double-stranded cDNA, which was inserted into the PstI site of pBR322 by oligo-dG:dC tailing. A recombinant plasmid containing carbonic anhydrase cDNA was identified by a positive hybridization selection assay and by partial DNA sequencing. Predicted amino acid sequences showed homology with the known sequences of rabbit and human carbonic anhydrase I and II. The clone contained sequences for most of the coding region and 600-700 base pairs at the 3' noncoding region of the mRNA. Hybridization analysis of poly(A+) RNA from uninduced and induced mouse erythroleukemic cells labeled for short and long time periods indicated that induction results in an increase of carbonic anhydrase mRNA in newly synthesized RNA. |
