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Publication : An electrophoretically detectable modification of glucosephosphate isomerase in mouse spermatozoa.

First Author  Buehr M Year  1981
Journal  J Reprod Fertil Volume  63
Issue  1 Pages  169-73
PubMed ID  7277315 Mgi Jnum  J:203030
Mgi Id  MGI:5523777 Doi  10.1530/jrf.0.0630169
Citation  Buehr M, et al. (1981) An electrophoretically detectable modification of glucosephosphate isomerase in mouse spermatozoa. J Reprod Fertil 63(1):169-73
abstractText  Cellulose acetate electrophoresis of glucosephosphate isomerase (GPI) from mouse testis and spermatozoa revealed a minor band which is not seen in electropherograms of GPI from other tissues. The minor band is associated equally with the two electrophoretic variants of GPI examined. Treatment of tissue extracts with various enzymes, enzyme inhibitors or thiol reagents failed to alter the appearance or position of the minor bands in sperm and testis GPI, or to induce their appearance in GPI from other tissues. No minor bands were seen in electropherograms of testis GPI from immature males, or from males whose testes lack spermatozoa for genetic reasons.
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