| First Author | Pellicano F | Year | 2006 |
| Journal | Gene Expr Patterns | Volume | 6 |
| Issue | 3 | Pages | 285-93 |
| PubMed ID | 16378758 | Mgi Jnum | J:105883 |
| Mgi Id | MGI:3616929 | Doi | 10.1016/j.modgep.2005.07.004 |
| Citation | Pellicano F, et al. (2006) Expression of coiled-coil protein 1, a novel gene downstream of FGF2, in the developing brain. Gene Expr Patterns 6(3):285-93 |
| abstractText | Fibroblast growth factor 2 (FGF2) plays an important role in cortical development. However, the genes downstream of FGF2 that mediate its effect are largely unknown. We have performed a microarray screening of genes regulated by FGF2 using primary cortical neuron culture derived from embryonic day 14.5 (E14.5) mouse forebrains. In this study, we have analysed a previously uncharacterised gene encoding a 180-amino acid protein, hereby named 'coiled-coil protein 1 (ccp1)', that showed a modest up-regulation upon FGF2 stimulation. Northern blots and RT-PCR showed specific expression of ccp1 in multiple tissues including adult and embryonic brains. In situ hybridizations revealed that ccp1 was expressed in the cortical plate between Reelin and Tbr1-positive layers in the dorsal cortex at E15.5. Furthermore, the expression pattern of ccp1 at E13.5-E14.5 reflected some of the aspects of tangential migration of cortical progenitors during the early phase. We observed that the expressed ccp1 protein was localised to endo/lysosomal compartment in the cell body as well as to vesicles present in the processes of primary cortical neurons and oligodendrocyte cell line. |
