| First Author | Dalit L | Year | 2022 |
| Journal | Immunol Cell Biol | Volume | 100 |
| Issue | 5 | Pages | 312-322 |
| PubMed ID | 35233830 | Mgi Jnum | J:345391 |
| Mgi Id | MGI:7579546 | Doi | 10.1111/imcb.12541 |
| Citation | Dalit L, et al. (2022) CXCL11 expressing C57BL/6 mice have intact adaptive immune responses to viral infection. Immunol Cell Biol 100(5):312-322 |
| abstractText | The chemokine receptor CXCR3 is expressed on immune cells to co-ordinate lymphocyte activation and migration. CXCR3 binds three chemokine ligands, CXCL9, CXCL10 and CXCL11. These ligands display distinct expression patterns and ligand signaling biases; however, how each ligand functions individually and collaboratively is incompletely understood. CXCL9 and CXCL10 are considered pro-inflammatory chemokines during viral infection, while CXCL11 may induce a tolerizing state. The investigation of the individual role of CXCL11 in vivo has been hampered as C57BL/6 mice carry several mutations that result in a null allele. Here, CRISPR/Cas9 was used to correct these mutations on a C57BL/6 background. It was validated that CXCL11(KI) mice expressed CXCL11 protein in dendritic cells, spleen and lung. CXCL11(KI) mice were largely phenotypically indistinguishable from C57BL/6 mice, both at steady-state and during two models of viral infection. While CXCL11 expression did not modify acute antiviral responses, this study provides a new tool to understand the role of CXCL11 in other experimental settings. |
