| First Author | Hashimoto Y | Year | 1998 |
| Journal | J Exp Med | Volume | 187 |
| Issue | 3 | Pages | 289-96 |
| PubMed ID | 9449709 | Mgi Jnum | J:45694 |
| Mgi Id | MGI:1195849 | Doi | 10.1084/jem.187.3.289 |
| Citation | Hashimoto Y, et al. (1998) Expression of the Elm1 gene, a novel gene of the CCN (connective tissue growth factor, Cyr61/Cef10, and neuroblastoma overexpressed gene) family, suppresses In vivo tumor growth and metastasis of K-1735 murine melanoma cells. J Exp Med 187(3):289-96 |
| abstractText | We previously isolated a partial cDNA fragment of a novel gene, Elm1 (expressed in low-metastatic cells), that is expressed in low-metastatic but not in high-metastatic K- 1735 mouse melanoma cells. Here we determined the full- length cDNA structure of Elm1 and investigated the effect of Elm1 expression on growth and metastatic potential of K- 1735 cells. The Elm1 gene encodes a predicted protein of 367 amino acids showing similar to 40% amino acid identity with the CCN (connective tissue growth factor [CTGF], Cyr61/Cef10, neuroblastoma overexpressed gene [Nov]) family proteins, which consist of secreted cysteine-rich proteins with growth regulatory functions. Elm1 is also a cysteine-rich protein and contains a signal peptide and four domains conserved in the CCN family proteins. Elm1 was highly conserved, expressed ubiquitously in diverse organs, and mapped to mouse chromosome 15. High-metastatic K-1735 M-2 cells, which did not express Elm1, were transfected with an Elm1 expression vector, and several stable clones with Elm1 expression were established. The in vivo growth rates of cells expressing a high level of Elm1 were remarkably slower than those of cells expressing a low level of Elm1. Metastatic potential of transfectants was reduced in proportion to the level of Elm1 expression. Thus, Elm1 is a novel gene of CCN family that can suppress the in vivo growth and metastatic potential of K-1735 mouse melanoma cells. |
