| First Author | Northrop JP | Year | 1994 |
| Journal | Nature | Volume | 369 |
| Issue | 6480 | Pages | 497-502 |
| PubMed ID | 8202141 | Mgi Jnum | J:22686 |
| Mgi Id | MGI:70544 | Doi | 10.1038/369497a0 |
| Citation | Northrop JP, et al. (1994) NF-AT components define a family of transcription factors targeted in T-cell activation [see comments]. Nature 369(6480):497-502 |
| abstractText | The NF-AT transcription complex is required for the expression of a group of proteins that collectively coordinate the immune response. Here we purify two proteins encoded by separate genes that represent the pre-existing (p) and cytosolic (c) components of NF-AT. Expression of the full-length complementary DNA encoding NF-ATc activates the interleukin (IL-2) promoter in non-T lymphocytes, whereas a dominant negative of NF-ATc specifically blocks activation of the IL-2 promoter in T lymphocytes, indicating that NF-ATc is required for IL-2 gene expression. NF-ATc RNA expression is largely restricted to lymphoid tissues and is induced upon T-cell activation. The other protein, NF-ATp, is highly homologous to NF-ATc over a limited domain which shows similarity to the Dorsal/Rel family, but has a wider tissue distribution. Agents that increase intracellular Ca2+ or activate protein kinase C independently modify NF-ATc, indicating that distinct signalling pathways converge on NF-ATc to regulate its function. |
