| First Author | Migliaccio AR | Year | 1993 |
| Journal | J Cell Physiol | Volume | 157 |
| Issue | 1 | Pages | 158-63 |
| PubMed ID | 7691834 | Mgi Jnum | J:14726 |
| Mgi Id | MGI:62889 | Doi | 10.1002/jcp.1041570120 |
| Citation | Migliaccio AR, et al. (1993) Induction of the murine W phenotype in long-term cultures of human cord blood cells by c-kit antisense oligomers. J Cell Physiol 157(1):158-63 |
| abstractText | The murine white (W) spotting locus is the site of the c-kit gene and encodes a tyrosine kinase receptor while the complementary Steel (Sl) locus encodes its ligand. Mutations at either locus have profound effects on hematopoiesis, particularly erythroid and mast cell proliferation. We added c-kit antisense oligonucleotides to long-term suspension cultures of enriched human umbilical cord progenitor cells. This resulted in the suppression of c-kit gene expression and the preferential suppression of the generation of erythroid burst-forming cells (BFU-E) which extended over the life of the culture (3 weeks). The results provide an in vitro model of the W phenotype in human hematopoiesis and confirm the importance of c-kit gene function in early erythropoiesis. Because the generation of BFU-E was suppressed even after c-kit gene expression had recovered, this gene product may be critical to the erythroid commitment process. |
