| First Author | Lo YF | Year | 1979 |
| Journal | Biochem Genet | Volume | 17 |
| Issue | 7-8 | Pages | 737-46 |
| PubMed ID | 540017 | Mgi Jnum | J:6290 |
| Mgi Id | MGI:54767 | Doi | 10.1007/BF00502132 |
| Citation | Lo YF, et al. (1979) Developmental expression of murine HPRT. I. Activities, heat stabilities, and electrophoretic mobilities in adult tissues. Biochem Genet 17(7-8):737-46 |
| abstractText | Total and specific activity of the enzyme hypoxanthine phosphoribosyltransferase (HPRT) varied widely among six tissues from C3H/f mice; the highest levels of activity were in brain. More striking were thermostability differences in tissue enzymes. Although brain, spleen, and kidney HPRT retained 65% basal activity after 15 min at 85 C, heart, liver, and erythrocyte HPRT retained only 20-30% initial activity. Kidney HPRT behaved as monospecific heat-stable enzyme (K-denatauration=0.022/min, and liver enzyme behaved as monospecific heat-labile enzyme (K-denaturation=0.061/min), while other tissues appeared to contain both forms of the enzyme. Multiple electrophoretic activity bands were present in all tissues; no activity band was restricted to a single tissue. The data presented here are consistent with the hypothesis that the distinct tissue properties of HPRT result from posttranslational modification of the product of a single genetic locus which is expressed in all tissues. |
