| First Author | Rayapureddi JP | Year | 2003 |
| Journal | Nature | Volume | 426 |
| Issue | 6964 | Pages | 295-8 |
| PubMed ID | 14628052 | Mgi Jnum | J:86608 |
| Mgi Id | MGI:2680856 | Doi | 10.1038/nature02093 |
| Citation | Rayapureddi JP, et al. (2003) Eyes absent represents a class of protein tyrosine phosphatases. Nature 426(6964):295-8 |
| abstractText | The Eyes absent proteins are members of a conserved regulatory network implicated in the development of the eye, muscle, kidney and ear. Mutations in the Eyes absent genes have been associated with several congenital disorders including the multi-organ disease bronchio-oto-renal syndrome, congenital cataracts and late-onset deafness. On the basis of previous analyses it has been shown that Eyes absent is a nuclear transcription factor, acting through interaction with homeodomain-containing Sine oculis (also known as Six) proteins. Here we show that Eyes absent is also a protein tyrosine phosphatase. It does not resemble the classical tyrosine phosphatases that use cysteine as a nucleophile and proceed by means of a thiol-phosphate intermediate. Rather, Eyes absent is the prototype for a class of protein tyrosine phosphatases that use a nucleophilic aspartic acid in a metal-dependent reaction. Furthermore, the phosphatase activity of Eyes absent contributes to its ability to induce eye formation in Drosophila. |
