Primary Identifier | MGI:6150835 | Allele Type | Targeted |
Attribute String | Conditional ready | Gene | Pgp |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site was inserted upstream of exon 1 and an FRT site flanked neomycin resistance gene cassette and a second loxP site downstream of exon 2. Also inserted downstream of exon 2 was a cDNA for the endogenous gene where a point mutation was engineered to change aspartate codon 34 into an asparagine codon (p.D34N or p.Asp34Asn). The neo cassette was removed through subsequent flp-mediated recombination. This leaves a conditional-ready allele with the entire gene (exons 1-2) floxed. Only after cre-mediated deletion of the endogenous gene will the mutated cDNA, which codes for a kinase-dead version of the protein, be expressed. |