| First Author | McCracken S | Year | 1997 |
| Journal | Genes Dev | Volume | 11 |
| Issue | 24 | Pages | 3306-18 |
| PubMed ID | 9407024 | Mgi Jnum | J:50704 |
| Mgi Id | MGI:1327624 | Doi | 10.1101/gad.11.24.3306 |
| Citation | McCracken S, et al. (1997) 5'-Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase II [see comments]. Genes Dev 11(24):3306-18 |
| abstractText | We have investigated the role of the RNA Polymerase II (Pol II) carboxy-terminal domain (CTD) in mRNA 5' capping. Transcripts made in vivo by Pol II with a truncated CTD had a lower proportion of capped 5' ends than those made by Pol II with a full-length CTD. In addition, the enzymes responsible for cap synthesis, RNA guanylyltransferase, and RNA (guanine-7)-methyl-transferase bound directly to the phosphorylated, but not to the nonphosphorylated, form of the CTD in vitro. These results suggest that: (1) Pol II-specific capping of nascent transcripts in vivo is enhanced by recruitment of the capping enzymes to the CTD and (2) capping is co-ordinated with CTD phosphorylation. |
