Primary Identifier | MGI:5905817 | Allele Type | Endonuclease-mediated |
Attribute String | Null/knockout | Gene | Chaer1 |
Strain of Origin | C57BL/6 | Is Recombinase | false |
Is Wild Type | false |
molecularNote | This knockout allele was created by deleting exon 2 (which represents the bulk of the gene) using CRISPR/Cas9 technology. The sgRNAs flanking the exon were 5'-GTAGTTTGCACGTTAGCAAG-3' upstream and 5'-GATTACCGAGAACTGCCCGA-3' downstream. |