First Author | Harigaya T | Year | 1996 |
Journal | Zoolog Sci | Volume | 13 |
Issue | 5 | Pages | 665-8 |
PubMed ID | 9004555 | Mgi Jnum | J:37981 |
Mgi Id | MGI:85374 | Doi | 10.2108/zsj.13.665 |
Citation | Harigaya T, et al. (1996) The mRNA expression of neurocan, a brain-specific chondroitin sulfate proteoglycan, in neoplastic mammary glands in mice. Zoolog Sci 13(5):665-8 |
abstractText | In the experiment of mouse transforming growth factor alpha (TGF alpha) gene expression in mammary tumors, various sizes of amplified products by reverse transcriptase-polymerase chain reaction (RT-PCR) using mouse TGF alpha primers were detected in addition to a predicted size in four strains of mice. During the further analysis of these RT-PCR products in mouse mammary tumors, the transcript of neurocan gene was detected in the mammary tumor from SHN mice by the cloning and nucleotide sequence analysis after RT-PCR reaction using mouse TGF alpha primers. The 5'-nucleotide sequence of sequential 246bp in the amplified cDNA of 527bp was completely identical to a middle part of mouse neurocan cDNA sequence, one of the chondroitin-sulfate proteoglycan expressed in the nervous tissue. |