| Primary Identifier | MGI:6724093 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Mest |
| Transmission | Germline | Strain of Origin | C57BL/6 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting vector contains a loxP-flanked neomycin cassette introduced to intron 2 and a loxP site in intron 3. Cre-mediated recombination removed exon 3 and the neo cassette. Deletion of exon 3 causes a frameshift mutation and the generation of a stop codon in exon 4. qRT-PCR analysis in adipose tissue shows complete loss of mRNA. |
