| First Author | Redell JB | Year | 1998 |
| Journal | J Biol Chem | Volume | 273 |
| Issue | 35 | Pages | 22807-18 |
| PubMed ID | 9712915 | Mgi Jnum | J:49646 |
| Mgi Id | MGI:1277788 | Doi | 10.1074/jbc.273.35.22807 |
| Citation | Redell JB, et al. (1998) Multiple promoter elements interact to control the transcription of the potassium channel gene, KCNJ2. J Biol Chem 273(35):22807-18 |
| abstractText | Potassium channels play important roles in shaping the electrical properties of excitable cells. Toward understanding the transcriptional regulation of a member of the inwardly rectifying potassium channel family, we have characterized the genomic structure and 5'-proximal promoter of the murine Kcnj2 gene (also referred to as IRK1 and Kir2.1). The Kcnj2 transcription unit is composed of two exons separated by a 5.5-kilobase pair intron. Deletion analysis of 5'-flanking sequences identified a promiscuously active 172-base pair minimal promoter, whereas expression from a construct containing additional upstream sequences was cell type-restricted. The minimal promoter contained an E box, a Y box, and three GC box consensus elements but lacked both TATA and CCAAT box elements. The activity of the minimal promoter was found to be controlled by a combination of the activities of the transcription factors Sp1, Sp3, and NF-Y. The interplay between Sp1, Sp3, and NF-Y within the architecture of the Kcnj2 promoter, the ubiquitous nature of these trans-acting factors, and the action of tissue-selective repressor element(s) may combine to enable a wide variety of cell types to differentially regulate Kcnj2 expression through transcriptional control. |
