| Primary Identifier | MGI:5566702 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Rgs8 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell line = EB3, a mutant E14TG2a line (129P2/OlaHsd). Targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette inactivated the Pou5f1 allele. J:208753 does not mention whether the null allele of Pou5f1 is also present in the line of mice created. |
| molecularNote | A floxed neomycin resistance cassette was inserted upstream of exon 4. An additional loxP site was inserted downstream of exon 5. Cre-mediated recombination removed the selection cassette, exon 4 and exon 5. Western blot analysis confirmed the absence of protein expression in the cerebellum. |
