| First Author | Nicoll DA | Year | 1990 |
| Journal | Science | Volume | 250 |
| Issue | 4980 | Pages | 562-5 |
| PubMed ID | 1700476 | Mgi Jnum | J:47389 |
| Mgi Id | MGI:1203375 | Doi | 10.1126/science.1700476 |
| Citation | Nicoll DA, et al. (1990) Molecular cloning and functional expression of the cardiac sarcolemmal Na(+)-Ca2+ exchanger. Science 250(4980):562-5 |
| abstractText | The Na(+)-Ca2+ exchanger of the cardiac sarcolemma can rapidly transport Ca2+ during excitation-contraction coupling. To begin molecular studies of this transporter, polyclonal antibodies were used to identify a complementary DNA (cDNA) clone encoding the Na(+)-Ca2+ exchanger protein. The cDNA hybridizes with a 7-kilobase RNA on a Northern blot and has an open reading frame of 970 amino acids. Hydropathy analysis suggests that the protein has multiple transmembrane helices, and a small region of the sequence is similar to that of the Na(+)- and K(+)-dependent adenosine triphosphatase. Polyclonal antibodies to a synthetic peptide from the deduced amino acid sequence react with sarcolemmal proteins of 70, 120, and 160 kilodaltons on immunoblots. RNA, synthesized from the cDNA clone, induces expression of Na(+)-Ca2+ exchange activity when injected into Xenopus oocytes. |
